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Primer Express Software v3. Download Primer Express Software v3. This minimizes the need for assay optimization and streamlines the design process.
Default and adjustable parameters Figure 1 provide the flexibility required for all users. Whether your research includes gene quantitation or allelic discrimination studies, Primer Express Software belongs in your lab. If you know the sequence, you need only to import or copy and paste the sequence and quickly view your results.
Alternatively, if you prefer to design the primers and probes manually, the procedure is equally simple and straight-forward as well. These guidelines are such that traditional variables, including magnesium chloride concentration and the thermal cycling protocol, should be standardized by using a master mix of reagents enzyme, salts, buffer and tailored primer and probe design.
This approach substantially reduces assay development time and improves laboratory efficiency by allowing multiple assays per plate to run simultaneously as all require the same conditions. For Research Use Only. Not for use in diagnostic procedures. Zur Verwendung mit Anwendung.
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